Hdl Handle:
http://hdl.handle.net/10675.2/621763
Title:
SYNTHESIS OF PEPTIDES WITH FLUORESCENT TAGS
Authors:
Weather, Angel; Sweatman, Zachary; Bae, Junsoo; Lebedyeva, Iryna
Abstract:
Covalently linked acceptor-spacer-donor (A-S-D) multichromophoric systems have previously been discussed largely in mechanistic terms, with emphasis on the roles of electronic and nuclear functions in determining the factors of internal electron transfer. Electron energy transfer between donor (D) and acceptor (A) molecules has long been applied to measure or estimate distance in macromolecular systems, molecular stacking, intramolecular changes caused by ionic strength or target molecule binding, to define protein folding and aggregation and finally to give information about conformational changes in the molecule. Coumarins represent a family of dyes that often play the role of electron donor in electron-charged systems. During tailoring of new chromophore dyads, coumarin derivatives have been linked to fullerene, peptides, and dyes (benzimidazol benzopyrans). In particular, 7-methoxy-2-oxo-2H-chromene-3-carboxylic acid is a commonly used tag that provides strong fluorescence and acts as an electron-donor. In current work we are developing chiral systems of 10 Å and more in order to establish a structure map whenever the donor-acceptor fluorophore is conjugating with another system (e. g. peptides). Our team enhances the fluorescence of the two coumarin-based fluorophores by linking them to the N- and C-peptide moieties via 1,2,3-triazine linkers.
Affiliation:
Department of Chemistry and Physics
Issue Date:
21-Feb-2018
URI:
http://hdl.handle.net/10675.2/621763
Type:
Thesis; Poster Presentation
Language:
en_US
Description:
Presentation given at the 19th Annual Phi Kappa Phi Student Research and Fine Arts Conference
Appears in Collections:
19th Annual PKP Student Research and Fine Arts Conference: Posters

Full metadata record

DC FieldValue Language
dc.contributor.authorWeather, Angelen
dc.contributor.authorSweatman, Zacharyen
dc.contributor.authorBae, Junsooen
dc.contributor.authorLebedyeva, Irynaen
dc.date.accessioned2018-02-21T13:33:48Z-
dc.date.available2018-02-21T13:33:48Z-
dc.date.issued2018-02-21-
dc.identifier.urihttp://hdl.handle.net/10675.2/621763-
dc.descriptionPresentation given at the 19th Annual Phi Kappa Phi Student Research and Fine Arts Conferenceen
dc.description.abstractCovalently linked acceptor-spacer-donor (A-S-D) multichromophoric systems have previously been discussed largely in mechanistic terms, with emphasis on the roles of electronic and nuclear functions in determining the factors of internal electron transfer. Electron energy transfer between donor (D) and acceptor (A) molecules has long been applied to measure or estimate distance in macromolecular systems, molecular stacking, intramolecular changes caused by ionic strength or target molecule binding, to define protein folding and aggregation and finally to give information about conformational changes in the molecule. Coumarins represent a family of dyes that often play the role of electron donor in electron-charged systems. During tailoring of new chromophore dyads, coumarin derivatives have been linked to fullerene, peptides, and dyes (benzimidazol benzopyrans). In particular, 7-methoxy-2-oxo-2H-chromene-3-carboxylic acid is a commonly used tag that provides strong fluorescence and acts as an electron-donor. In current work we are developing chiral systems of 10 Å and more in order to establish a structure map whenever the donor-acceptor fluorophore is conjugating with another system (e. g. peptides). Our team enhances the fluorescence of the two coumarin-based fluorophores by linking them to the N- and C-peptide moieties via 1,2,3-triazine linkers.en
dc.language.isoen_USen
dc.subjectPeptideen
dc.subjectFluoresceneen
dc.titleSYNTHESIS OF PEPTIDES WITH FLUORESCENT TAGSen_US
dc.typeThesisen
dc.typePoster Presentationen
dc.contributor.departmentDepartment of Chemistry and Physicsen
dc.description.advisorLebedyeva, Irynaen
dc.contributor.affiliationAugusta Universityen
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