Hdl Handle:
http://hdl.handle.net/10675.2/621759
Title:
Energy Transfer Phenomenon with Nanoluciferase as Energy Donor
Authors:
Bailey, Joseph
Abstract:
Bioluminescence is a phenomenon that occurs where light is emitted from a chemical reaction that occurs in an organism.An enzyme called a luciferase assists in the catalysis of a reaction that releases the energy of broken bonds as light at a certain wavelength. Bioluminescence Resonance Energy Transfer (BRET) is a technique that utilizes a luciferase as an energy donor, whichemits energy from catalysis of a substrate. Fluorescence by Unbound Excitation from Luminescence (FUEL) deviates fromBRET slightly. FUEL involves an energy donor exciting an energy accepting particle; however, the acceptor and donor are unattached to each other. The luciferase being used as an energy donor is Nanoluciferase (Nluc)that emits light at 454 nm. Nluc beenmodified to expressa His-6 tag. The energy acceptors being used are Alexa 488, 555, and 647 and reemit light at the corresponding wavelength. Each of the Alexa dyes has been modified to include an anti-His antibody, causing attachment between Nluc and the respective Alexa dye.Energy transfer in the form of BRET did occur between Nluc and the Alexa 488 and minimally with Alexa 555. No energy transfer was noted with Alexa 647. Energy transfer was quantified using a fluorometer.
Affiliation:
Department of Chemistry and Physics
Issue Date:
12-Feb-2018
URI:
http://hdl.handle.net/10675.2/621759
Submitted date:
25-JAN-2018 06:25PM
Type:
Poster Presentation
Description:
Presentation given at the 19th Annual Phi Kappa Phi Student Research and Fine Arts Conference
Appears in Collections:
19th Annual PKP Student Research and Fine Arts Conference: Posters

Full metadata record

DC FieldValue Language
dc.contributor.authorBailey, Josephen
dc.date.accessioned2018-02-12T17:19:36Z-
dc.date.available2018-02-12T17:19:36Z-
dc.date.issued2018-02-12-
dc.date.submitted25-JAN-2018 06:25PM-
dc.identifier.urihttp://hdl.handle.net/10675.2/621759-
dc.descriptionPresentation given at the 19th Annual Phi Kappa Phi Student Research and Fine Arts Conferenceen
dc.description.abstractBioluminescence is a phenomenon that occurs where light is emitted from a chemical reaction that occurs in an organism.An enzyme called a luciferase assists in the catalysis of a reaction that releases the energy of broken bonds as light at a certain wavelength. Bioluminescence Resonance Energy Transfer (BRET) is a technique that utilizes a luciferase as an energy donor, whichemits energy from catalysis of a substrate. Fluorescence by Unbound Excitation from Luminescence (FUEL) deviates fromBRET slightly. FUEL involves an energy donor exciting an energy accepting particle; however, the acceptor and donor are unattached to each other. The luciferase being used as an energy donor is Nanoluciferase (Nluc)that emits light at 454 nm. Nluc beenmodified to expressa His-6 tag. The energy acceptors being used are Alexa 488, 555, and 647 and reemit light at the corresponding wavelength. Each of the Alexa dyes has been modified to include an anti-His antibody, causing attachment between Nluc and the respective Alexa dye.Energy transfer in the form of BRET did occur between Nluc and the Alexa 488 and minimally with Alexa 555. No energy transfer was noted with Alexa 647. Energy transfer was quantified using a fluorometer.en
dc.subjectLuciferaseen
dc.subjectEnergy Transferen
dc.subjectLinkage typeen
dc.titleEnergy Transfer Phenomenon with Nanoluciferase as Energy Donoren
dc.typePoster Presentationen
dc.contributor.departmentDepartment of Chemistry and Physicsen
dc.contributor.sponsorSpencer, Angelaen
dc.contributor.sponsorDepartment of Chemistry and Physicsen
dc.contributor.affiliationAugusta Universityen
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