Hdl Handle:
http://hdl.handle.net/10675.2/621525
Title:
Human vs Machine: A Systematic Review of Automated Bead-Based Multiplex Immunoassay Performance
Authors:
Charles, Mosa; Gao, Jie; Jiang, Jingyuan; Cannon, Joseph; Sloan, Gloria; Stuart, Ansley ( 0000-0003-1154-0435 ) ; Weintraub, MJ
Abstract:
Autoimmune diseases are important contributors to morbidity and mortality in the United States. Researchers have identified more than 20 subtypes of antinuclear antibodies (ANAs), which are the hallmark of autoimmune diseases (1). Reliable, rapid ANA tests with acceptable sensitivity and specificity are in high demand for the timely diagnosis and treatment of autoimmune diseases (2,3). The gold standard for ANA detection is manual indirect immunofluorescence (IIF) with Hep-2 cells. This method is highly sensitive but has several flaws. It relies heavily on highly skilled morphologists which may lead to reader bias, and increased inter-laboratory variation (2,4). This study is a systematic review of the currently available multiplexed systems (i.e., Bioplex 2200, AtheNA Multi-Lyte ANA and FIDIS Connective 10) that might serve as alternative methods for detection of ANAs in the diagnosis of autoimmune diseases (5).
Affiliation:
College of Allied Health Sciences - Clinical Laboratory Sciences
Issue Date:
10-Mar-2017
URI:
http://hdl.handle.net/10675.2/621525
Type:
Presentation
Language:
en_US
Appears in Collections:
Department of Medical Laboratory, Imaging & Radiologic Sciences: Student Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorCharles, Mosaen
dc.contributor.authorGao, Jieen
dc.contributor.authorJiang, Jingyuanen
dc.contributor.authorCannon, Josephen
dc.contributor.authorSloan, Gloriaen
dc.contributor.authorStuart, Ansleyen
dc.contributor.authorWeintraub, MJen
dc.date.accessioned2017-08-07T13:58:26Z-
dc.date.available2017-08-07T13:58:26Z-
dc.date.issued2017-03-10-
dc.identifier.urihttp://hdl.handle.net/10675.2/621525-
dc.description.abstractAutoimmune diseases are important contributors to morbidity and mortality in the United States. Researchers have identified more than 20 subtypes of antinuclear antibodies (ANAs), which are the hallmark of autoimmune diseases (1). Reliable, rapid ANA tests with acceptable sensitivity and specificity are in high demand for the timely diagnosis and treatment of autoimmune diseases (2,3). The gold standard for ANA detection is manual indirect immunofluorescence (IIF) with Hep-2 cells. This method is highly sensitive but has several flaws. It relies heavily on highly skilled morphologists which may lead to reader bias, and increased inter-laboratory variation (2,4). This study is a systematic review of the currently available multiplexed systems (i.e., Bioplex 2200, AtheNA Multi-Lyte ANA and FIDIS Connective 10) that might serve as alternative methods for detection of ANAs in the diagnosis of autoimmune diseases (5).en
dc.language.isoen_USen
dc.subjectautomated bead-based multiplex immunoassayen
dc.subjectsystematic reviewen
dc.subjectmultiplex immunoassayen
dc.titleHuman vs Machine: A Systematic Review of Automated Bead-Based Multiplex Immunoassay Performanceen_US
dc.typePresentationen
dc.contributor.departmentCollege of Allied Health Sciences - Clinical Laboratory Sciencesen
dc.description.advisorJoseph Cannon and Ansley Stuarten
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