Obesity related methylation changes in DNA of peripheral blood leukocytes

Hdl Handle:
http://hdl.handle.net/10675.2/621
Title:
Obesity related methylation changes in DNA of peripheral blood leukocytes
Authors:
Wang, Xiaoling; Zhu, Haidong; Snieder, Harold; Su, Shaoyong; Munn, David H.; Harshfield, Gregory; Maria, Bernard L.; Dong, Yanbin; Treiber, Frank A.; Gutin, Bernard; Shi, Huidong
Abstract:
Background: Despite evidence linking obesity to impaired immune function, little is known about the specific mechanisms. Because of emerging evidence that immune responses are epigenetically regulated, we hypothesized that DNA methylation changes are involved in obesity induced immune dysfunction and aimed to identify these changes.; Method: We conducted a genome wide methylation analysis on seven obese cases and seven lean controls aged 14 to 18 years from extreme ends of the obesity distribution and performed further validation of six CpG sites from six genes in 46 obese cases and 46 lean controls aged 14 to 30 years.; Results: In comparison with the lean controls, we observed one CpG site in the UBASH3A gene showing higher methylation levels and one CpG site in the TRIM3 gene showing lower methylation levels in the obese cases in both the genome wide step (P = 5 Ã 10-6 and P = 2 Ã 10-5 for the UBASH3A and the TRIM3 gene respectively) and the validation step (P = 0.008 and P = 0.001 for the UBASH3A and the TRIM3 gene respectively).; Conclusions: Our results provide evidence that obesity is associated with methylation changes in blood leukocyte DNA. Further studies are warranted to determine the causal direction of this relationship as well as whether such methylation changes can lead to immune dysfunction.
Citation:
BMC Med. 2010 Dec 21; 8:87
Issue Date:
21-Dec-2010
URI:
http://hdl.handle.net/10675.2/621
DOI:
10.1186/1741-7015-8-87
PubMed ID:
21176133
PubMed Central ID:
PMC3016263
Type:
Article
ISSN:
1741-7015
Appears in Collections:
Georgia Prevention Center: Faculty Research and Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, Xiaolingen_US
dc.contributor.authorZhu, Haidongen_US
dc.contributor.authorSnieder, Harolden_US
dc.contributor.authorSu, Shaoyongen_US
dc.contributor.authorMunn, David H.en_US
dc.contributor.authorHarshfield, Gregoryen_US
dc.contributor.authorMaria, Bernard L.en_US
dc.contributor.authorDong, Yanbinen_US
dc.contributor.authorTreiber, Frank A.en_US
dc.contributor.authorGutin, Bernarden_US
dc.contributor.authorShi, Huidongen_US
dc.date.accessioned2012-10-26T16:26:54Z-
dc.date.available2012-10-26T16:26:54Z-
dc.date.issued2010-12-21en_US
dc.identifier.citationBMC Med. 2010 Dec 21; 8:87en_US
dc.identifier.issn1741-7015en_US
dc.identifier.pmid21176133en_US
dc.identifier.doi10.1186/1741-7015-8-87en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/621-
dc.description.abstractBackground: Despite evidence linking obesity to impaired immune function, little is known about the specific mechanisms. Because of emerging evidence that immune responses are epigenetically regulated, we hypothesized that DNA methylation changes are involved in obesity induced immune dysfunction and aimed to identify these changes.en_US
dc.description.abstractMethod: We conducted a genome wide methylation analysis on seven obese cases and seven lean controls aged 14 to 18 years from extreme ends of the obesity distribution and performed further validation of six CpG sites from six genes in 46 obese cases and 46 lean controls aged 14 to 30 years.en_US
dc.description.abstractResults: In comparison with the lean controls, we observed one CpG site in the UBASH3A gene showing higher methylation levels and one CpG site in the TRIM3 gene showing lower methylation levels in the obese cases in both the genome wide step (P = 5 Ã 10-6 and P = 2 Ã 10-5 for the UBASH3A and the TRIM3 gene respectively) and the validation step (P = 0.008 and P = 0.001 for the UBASH3A and the TRIM3 gene respectively).en_US
dc.description.abstractConclusions: Our results provide evidence that obesity is associated with methylation changes in blood leukocyte DNA. Further studies are warranted to determine the causal direction of this relationship as well as whether such methylation changes can lead to immune dysfunction.en_US
dc.rightsCopyright ©2010 Wang et al; licensee BioMed Central Ltd.en_US
dc.subjectResearch Articleen_US
dc.titleObesity related methylation changes in DNA of peripheral blood leukocytesen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC3016263en_US
dc.contributor.corporatenameGeorgia Institute for Prevention of Human Diseases and Accidents-

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