Hdl Handle:
http://hdl.handle.net/10675.2/62
Title:
Rab11 in dysplasia of Barrett's epithelia.
Authors:
Goldenring, J R; Ray, G S; Lee, J R
Abstract:
Barrett's esophagus predisposes affected patients to the development of esophageal adenocarcinoma. The development of adenocarcinoma proceeds along a progression through low- and high-grade dysplasia. Surveillance of Barrett's patients requires serial endoscopic investigations and grading mucosal biopsies. Unfortunately, grading of biopsies by conventional hematoxylin and eosin staining is fraught with significant interobserver variations. We have found in both biopsy and resection specimens that immunostaining for the small GTP binding protein Rab11 is increased in low-grade dysplastic cells. This staining is lost in high-grade dysplastic cells. These results suggest that low-grade dysplastic cells undergo an apical trafficking blockade, which is released as cells progress to the less differentiated phenotype of high-grade dysplasia and adenocarcinoma. Examination of the SKGT-4 esophageal adenocarcinoma cell line demonstrated prominent mRNA and protein expression for Rab11. Rab11 immunostaining was present in SKGT-4 cells as a perinuclear nidus of punctate staining along with a more diffuse punctate pattern. Thus, Rab11 expression was present in a esophageal adenocarcinoma cells in culture. Markers of vesicle trafficking may be critical factors for grading of mucosal dysplastic transitions leading to adenocarcinoma.
Citation:
Yale J Biol Med. 1999 Mar-Jun; 72(2-3):113-120
Issue Date:
16-May-2000
URI:
http://hdl.handle.net/10675.2/62
PubMed ID:
10780572
PubMed Central ID:
PMC2579020
Type:
Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.
ISSN:
0044-0086
Appears in Collections:
Institute of Molecular Medicine and Genetics: Faculty Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorGoldenring, J Ren_US
dc.contributor.authorRay, G Sen_US
dc.contributor.authorLee, J Ren_US
dc.date.accessioned2010-09-24T21:26:50Z-
dc.date.available2010-09-24T21:26:50Z-
dc.date.issued2000-05-16en_US
dc.identifier.citationYale J Biol Med. 1999 Mar-Jun; 72(2-3):113-120en_US
dc.identifier.issn0044-0086en_US
dc.identifier.pmid10780572en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/62-
dc.description.abstractBarrett's esophagus predisposes affected patients to the development of esophageal adenocarcinoma. The development of adenocarcinoma proceeds along a progression through low- and high-grade dysplasia. Surveillance of Barrett's patients requires serial endoscopic investigations and grading mucosal biopsies. Unfortunately, grading of biopsies by conventional hematoxylin and eosin staining is fraught with significant interobserver variations. We have found in both biopsy and resection specimens that immunostaining for the small GTP binding protein Rab11 is increased in low-grade dysplastic cells. This staining is lost in high-grade dysplastic cells. These results suggest that low-grade dysplastic cells undergo an apical trafficking blockade, which is released as cells progress to the less differentiated phenotype of high-grade dysplasia and adenocarcinoma. Examination of the SKGT-4 esophageal adenocarcinoma cell line demonstrated prominent mRNA and protein expression for Rab11. Rab11 immunostaining was present in SKGT-4 cells as a perinuclear nidus of punctate staining along with a more diffuse punctate pattern. Thus, Rab11 expression was present in a esophageal adenocarcinoma cells in culture. Markers of vesicle trafficking may be critical factors for grading of mucosal dysplastic transitions leading to adenocarcinoma.en_US
dc.rightsThe PMC Open Access Subset is a relatively small part of the total collection of articles in PMC. Articles in the PMC Open Access Subset are still protected by copyright, but are made available under a Creative Commons or similar license that generally allows more liberal redistribution and reuse than a traditional copyrighted work. Please refer to the license statement in each article for specific terms of use. The license terms are not identical for all articles in this subset.en_US
dc.subject.meshAdenocarcinoma / metabolism / pathologyen_US
dc.subject.meshBarrett Esophagus / metabolism / pathologyen_US
dc.subject.meshBiopsyen_US
dc.subject.meshEpithelium / metabolism / pathologyen_US
dc.subject.meshEsophageal Neoplasms / metabolism / pathologyen_US
dc.subject.meshHumansen_US
dc.subject.meshPrecancerous Conditions / metabolism / pathologyen_US
dc.subject.meshReceptor, Epidermal Growth Factor / metabolismen_US
dc.subject.meshStaining and Labeling / methodsen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.subject.meshrab GTP-Binding Proteins / analysis / metabolismen_US
dc.titleRab11 in dysplasia of Barrett's epithelia.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
dc.typeResearch Support, U.S. Gov't, Non-P.H.S.en_US
dc.typeResearch Support, U.S. Gov't, P.H.S.en_US
dc.identifier.pmcidPMC2579020en_US
dc.contributor.corporatenameInstitute of Molecular Medicine and Geneticsen_US

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