Differential effects of taurine treatment and taurine deficiency on the outcome of renal ischemia reperfusion injury

Hdl Handle:
http://hdl.handle.net/10675.2/612
Title:
Differential effects of taurine treatment and taurine deficiency on the outcome of renal ischemia reperfusion injury
Authors:
Mozaffari, Mahmood S.; Abdelsayed, Rafik; Patel, Champa; Wimborne, Hereward J. C.; Liu, Jun Yao; Schaffer, Stephen W
Abstract:
Taurine possesses membrane stabilization, osmoregulatory and antioxidant properties, aspects of relevance to ischemic injury. We tested the hypothesis that body taurine status is a determinant of renal ischemic injury. Accordingly, renal function and structure were examined in control (C), taurine-treated (TT) and taurine deficient (TD) rats that were subjected to bilateral renal ischemia (60 min) followed by reperfusion (IR); sham operated rats served as controls. Baseline urine osmolality was greater in the TD group than in the control and the TT groups, an effect associated with increased renal aquaporin 2 level. The IR insult reduced urine osmolality (i.e., day-1 post insult); the TD/IR group displayed a more marked recovery in urine osmolality by day-6 post insult than the other two groups. Fluid and sodium excretions were lower in the TD/IR group, suggesting propensity to retention. Histopathological examination revealed the presence of tubular necrotic foci in the C/IR group than sham controls. While renal architecture of the TD/IR group showed features resembling sham controls, the TT/IR group showed dilated tubules, which lacked immunostaining for aquaporin 2, but not 1, suggestive of proximal tubule origin. Finally, assessment of cell proliferation and apoptosis revealed lower proliferation but higher apoptotic foci in the TT/IR group than other IR groups. Collectively, the results indicate that body taurine status is a major determinant of renal IR injury.
Citation:
J Biomed Sci. 2010 Aug 24; 17(Suppl 1):S32
Issue Date:
24-Aug-2010
URI:
http://hdl.handle.net/10675.2/612
DOI:
10.1186/1423-0127-17-S1-S32
PubMed ID:
20804608
PubMed Central ID:
PMC2994366
Type:
Article
ISSN:
1423-0127
Appears in Collections:
Department of Oral Biology Faculty Papers

Full metadata record

DC FieldValue Language
dc.contributor.authorMozaffari, Mahmood S.en_US
dc.contributor.authorAbdelsayed, Rafiken_US
dc.contributor.authorPatel, Champaen_US
dc.contributor.authorWimborne, Hereward J. C.en_US
dc.contributor.authorLiu, Jun Yaoen_US
dc.contributor.authorSchaffer, Stephen Wen_US
dc.date.accessioned2012-10-26T16:26:52Z-
dc.date.available2012-10-26T16:26:52Z-
dc.date.issued2010-08-24en_US
dc.identifier.citationJ Biomed Sci. 2010 Aug 24; 17(Suppl 1):S32en_US
dc.identifier.issn1423-0127en_US
dc.identifier.pmid20804608en_US
dc.identifier.doi10.1186/1423-0127-17-S1-S32en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/612-
dc.description.abstractTaurine possesses membrane stabilization, osmoregulatory and antioxidant properties, aspects of relevance to ischemic injury. We tested the hypothesis that body taurine status is a determinant of renal ischemic injury. Accordingly, renal function and structure were examined in control (C), taurine-treated (TT) and taurine deficient (TD) rats that were subjected to bilateral renal ischemia (60 min) followed by reperfusion (IR); sham operated rats served as controls. Baseline urine osmolality was greater in the TD group than in the control and the TT groups, an effect associated with increased renal aquaporin 2 level. The IR insult reduced urine osmolality (i.e., day-1 post insult); the TD/IR group displayed a more marked recovery in urine osmolality by day-6 post insult than the other two groups. Fluid and sodium excretions were lower in the TD/IR group, suggesting propensity to retention. Histopathological examination revealed the presence of tubular necrotic foci in the C/IR group than sham controls. While renal architecture of the TD/IR group showed features resembling sham controls, the TT/IR group showed dilated tubules, which lacked immunostaining for aquaporin 2, but not 1, suggestive of proximal tubule origin. Finally, assessment of cell proliferation and apoptosis revealed lower proliferation but higher apoptotic foci in the TT/IR group than other IR groups. Collectively, the results indicate that body taurine status is a major determinant of renal IR injury.en_US
dc.rightsCopyright ©2010 Mozaffari et al; licensee BioMed Central Ltd.en_US
dc.subjectReviewen_US
dc.subject.meshAnimalsen_US
dc.subject.meshApoptosisen_US
dc.subject.meshAquaporin 2en_US
dc.subject.meshCell Proliferationen_US
dc.subject.meshKidneyen_US
dc.subject.meshKidney Diseasesen_US
dc.subject.meshKidney Function Testsen_US
dc.subject.meshMaleen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReperfusion Injuryen_US
dc.subject.meshTaurineen_US
dc.subject.meshTreatment Outcomeen_US
dc.titleDifferential effects of taurine treatment and taurine deficiency on the outcome of renal ischemia reperfusion injuryen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC2994366en_US
dc.contributor.corporatenameDepartment of Oral Biology-
dc.contributor.corporatenameDepartment of Oral Health and Diagnostic Sciences-

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