Hdl Handle:
http://hdl.handle.net/10675.2/601068
Title:
Cloning, Purification, and Inhibition of the SUV39H2 Histone Methyl Transferase
Authors:
Jones, Preston Dimitri
Abstract:
The most significant challenge in treating human colorectal cancer (CRC) is metastasis of the cancer. Although the study of colorectal cancer metastasis is still elementary, it is known that silencing of Fas expression is a hallmark of human colorectal cancer metastasis. The Fas protein is a member of the tumor necrosis factor (TNF)- a receptor superfamily that plays a major role in the regulation of programmed cell death. The interaction of Fas with its ligand, Fas L, triggers a signal cascade that ends in apoptosis. Silencing of Fas expression thus allows cancer cells to evade cell death triggered by the immune system. In human CRC patients, the Fas promoter is characterized by high H3K9 trimethylation (H3K9me3). This methylation is primarily caused by histone methyl transferases such as SUV39H2. We hypothesize that inhibiting H3K9 trimethylation is a potential anti-cancer strategy. To study this possibility, the gene for human SUV39H2 was cloned into pET21 and the protein was overexpressed in E.coli BL21- DE3 cells. The activity of the purified protein will be tested against various inhibitors. Funding Source: National Science Foundation
Affiliation:
Department of Chemistry and Physics
Issue Date:
Mar-2016
URI:
http://hdl.handle.net/10675.2/601068
Type:
Presentation
Language:
en_US
Description:
Presentation given at the 17th Annual Phi Kappa Phi Student Research and Fine Arts Conference
Appears in Collections:
17th Annual Phi Kappa Phi Student Research and Fine Arts Conference: Oral Symposia II

Full metadata record

DC FieldValue Language
dc.contributor.authorJones, Preston Dimitrien
dc.date.accessioned2016-03-09T21:01:43Zen
dc.date.available2016-03-09T21:01:43Zen
dc.date.issued2016-03en
dc.identifier.urihttp://hdl.handle.net/10675.2/601068en
dc.descriptionPresentation given at the 17th Annual Phi Kappa Phi Student Research and Fine Arts Conferenceen
dc.description.abstractThe most significant challenge in treating human colorectal cancer (CRC) is metastasis of the cancer. Although the study of colorectal cancer metastasis is still elementary, it is known that silencing of Fas expression is a hallmark of human colorectal cancer metastasis. The Fas protein is a member of the tumor necrosis factor (TNF)- a receptor superfamily that plays a major role in the regulation of programmed cell death. The interaction of Fas with its ligand, Fas L, triggers a signal cascade that ends in apoptosis. Silencing of Fas expression thus allows cancer cells to evade cell death triggered by the immune system. In human CRC patients, the Fas promoter is characterized by high H3K9 trimethylation (H3K9me3). This methylation is primarily caused by histone methyl transferases such as SUV39H2. We hypothesize that inhibiting H3K9 trimethylation is a potential anti-cancer strategy. To study this possibility, the gene for human SUV39H2 was cloned into pET21 and the protein was overexpressed in E.coli BL21- DE3 cells. The activity of the purified protein will be tested against various inhibitors. Funding Source: National Science Foundationen
dc.language.isoen_USen
dc.subjectColorectal Neoplasmsen
dc.subjectNeoplasm Metastasisen
dc.titleCloning, Purification, and Inhibition of the SUV39H2 Histone Methyl Transferaseen_US
dc.typePresentationen
dc.contributor.departmentDepartment of Chemistry and Physicsen
dc.description.advisorSpencer, Angelaen
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