Molecular Composition of Staufen2-Containing Ribonucleoproteins in Embryonic Rat Brain

Hdl Handle:
http://hdl.handle.net/10675.2/589
Title:
Molecular Composition of Staufen2-Containing Ribonucleoproteins in Embryonic Rat Brain
Authors:
Maher-Laporte, Marjolaine; Berthiaume, Frederic; Moreau, Mireille; Julien, Louis-Andre; Lapointe, Gabriel; Mourez, Michael; DesGroseillers, Luc
Abstract:
Messenger ribonucleoprotein particles (mRNPs) are used to transport mRNAs along neuronal dendrites to their site of translation. Numerous mRNA-binding and regulatory proteins within mRNPs finely regulate the fate of bound-mRNAs. Their specific combination defines different types of mRNPs that in turn are related to specific synaptic functions. One of these mRNA-binding proteins, Staufen2 (Stau2), was shown to transport dendritic mRNAs along microtubules. Its knockdown expression in neurons was shown to change spine morphology and synaptic functions. To further understand the molecular mechanisms by which Stau2 modulates synaptic function in neurons, it is important to identify and characterize protein co-factors that regulate the fate of Stau2-containing mRNPs. To this end, a proteomic approach was used to identify co-immunoprecipitated proteins in Staufen2-containing mRNPs isolated from embryonic rat brains. The proteomic approach identified mRNA-binding proteins (PABPC1, hnRNP H1, YB1 and hsc70), proteins of the cytoskeleton (a- and b-tubulin) and RUFY3 a poorly characterized protein. While PABPC1 and YB1 associate with Stau2-containing mRNPs through RNAs, hsc70 is directly bound to Stau2 and this interaction is regulated by ATP. PABPC1 and YB1 proteins formed puncta in dendrites of embryonic rat hippocampal neurons. However, they poorly co-localized with Stau2 in the large dendritic complexes suggesting that they are rather components of Stau2-containing mRNA particles. All together, these results represent a further step in the characterization of Stau2-containing mRNPs in neurons and provide new tools to study and understand how Stau2-containing mRNPs are transported, translationally silenced during transport and/or locally expressed according to cell needs.
Editors:
Mei, Lin
Citation:
PLoS One. 2010 Jun 28; 5(6):e11350
Issue Date:
28-Jun-2010
URI:
http://hdl.handle.net/10675.2/589
DOI:
10.1371/journal.pone.0011350
PubMed ID:
20596529
PubMed Central ID:
PMC2893162
Type:
Article
ISSN:
1932-6203
Appears in Collections:
Department of Neurology: Faculty Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorMaher-Laporte, Marjolaineen_US
dc.contributor.authorBerthiaume, Fredericen_US
dc.contributor.authorMoreau, Mireilleen_US
dc.contributor.authorJulien, Louis-Andreen_US
dc.contributor.authorLapointe, Gabrielen_US
dc.contributor.authorMourez, Michaelen_US
dc.contributor.authorDesGroseillers, Lucen_US
dc.contributor.editorMei, Lin-
dc.date.accessioned2012-10-26T16:26:48Z-
dc.date.available2012-10-26T16:26:48Z-
dc.date.issued2010-06-28en_US
dc.identifier.citationPLoS One. 2010 Jun 28; 5(6):e11350en_US
dc.identifier.issn1932-6203en_US
dc.identifier.pmid20596529en_US
dc.identifier.doi10.1371/journal.pone.0011350en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/589-
dc.description.abstractMessenger ribonucleoprotein particles (mRNPs) are used to transport mRNAs along neuronal dendrites to their site of translation. Numerous mRNA-binding and regulatory proteins within mRNPs finely regulate the fate of bound-mRNAs. Their specific combination defines different types of mRNPs that in turn are related to specific synaptic functions. One of these mRNA-binding proteins, Staufen2 (Stau2), was shown to transport dendritic mRNAs along microtubules. Its knockdown expression in neurons was shown to change spine morphology and synaptic functions. To further understand the molecular mechanisms by which Stau2 modulates synaptic function in neurons, it is important to identify and characterize protein co-factors that regulate the fate of Stau2-containing mRNPs. To this end, a proteomic approach was used to identify co-immunoprecipitated proteins in Staufen2-containing mRNPs isolated from embryonic rat brains. The proteomic approach identified mRNA-binding proteins (PABPC1, hnRNP H1, YB1 and hsc70), proteins of the cytoskeleton (a- and b-tubulin) and RUFY3 a poorly characterized protein. While PABPC1 and YB1 associate with Stau2-containing mRNPs through RNAs, hsc70 is directly bound to Stau2 and this interaction is regulated by ATP. PABPC1 and YB1 proteins formed puncta in dendrites of embryonic rat hippocampal neurons. However, they poorly co-localized with Stau2 in the large dendritic complexes suggesting that they are rather components of Stau2-containing mRNA particles. All together, these results represent a further step in the characterization of Stau2-containing mRNPs in neurons and provide new tools to study and understand how Stau2-containing mRNPs are transported, translationally silenced during transport and/or locally expressed according to cell needs.en_US
dc.rightsMaher-Laporte et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.subjectResearch Articleen_US
dc.subjectNeuroscienceen_US
dc.subjectBiochemistry/Cell Signaling and Trafficking Structuresen_US
dc.subjectMolecular Biology/mRNA Transport and Localizationen_US
dc.subjectMolecular Biology/Post-Translational Regulation of Gene Expressionen_US
dc.titleMolecular Composition of Staufen2-Containing Ribonucleoproteins in Embryonic Rat Brainen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC2893162en_US
dc.contributor.corporatenameDepartment of Neurology-
dc.contributor.corporatenameCollege of Graduate Studies-

Related articles on PubMed

All Items in Scholarly Commons are protected by copyright, with all rights reserved, unless otherwise indicated.