Hdl Handle:
http://hdl.handle.net/10675.2/587
Title:
Neurexin in Embryonic Drosophila Neuromuscular Junctions
Authors:
Chen, Kaiyun; Gracheva, Elena O.; Yu, Szi-Chieh; Sheng, Qi; Richmond, Janet; Featherstone, David E.
Abstract:
Background: Neurexin is a synaptic cell adhesion protein critical for synapse formation and function. Mutations in neurexin and neurexin-interacting proteins have been implicated in several neurological diseases. Previous studies have described Drosophila neurexin mutant phenotypes in third instar larvae and adults. However, the expression and function of Drosophila neurexin early in synapse development, when neurexin function is thought to be most important, has not been described.; Methodology/Principal Findings: We use a variety of techniques, including immunohistochemistry, electron microscopy, in situ hybridization, and electrophysiology, to characterize neurexin expression and phenotypes in embryonic Drosophila neuromuscular junctions (NMJs). Our results surprisingly suggest that neurexin in embryos is present both pre and postsynaptically. Presynaptic neurexin promotes presynaptic active zone formation and neurotransmitter release, but along with postsynaptic neurexin, also suppresses formation of ectopic glutamate receptor clusters. Interestingly, we find that loss of neurexin only affects receptors containing the subunit GluRIIA.; Conclusions/Significance: Our study extends previous results and provides important detail regarding the role of neurexin in Drosophila glutamate receptor abundance. The possibility that neurexin is present postsynaptically raises new hypotheses regarding neurexin function in synapses, and our results provide new insights into the role of neurexin in synapse development.
Editors:
Mei, Lin
Citation:
PLoS One. 2010 Jun 14; 5(6):e11115
Issue Date:
14-Jun-2010
URI:
http://hdl.handle.net/10675.2/587
DOI:
10.1371/journal.pone.0011115
PubMed ID:
20559439
PubMed Central ID:
PMC2885429
Type:
Article
ISSN:
1932-6203
Appears in Collections:
Department of Neurology: Faculty Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorChen, Kaiyunen_US
dc.contributor.authorGracheva, Elena O.en_US
dc.contributor.authorYu, Szi-Chiehen_US
dc.contributor.authorSheng, Qien_US
dc.contributor.authorRichmond, Janeten_US
dc.contributor.authorFeatherstone, David E.en_US
dc.contributor.editorMei, Lin-
dc.date.accessioned2012-10-26T16:26:48Z-
dc.date.available2012-10-26T16:26:48Z-
dc.date.issued2010-06-14en_US
dc.identifier.citationPLoS One. 2010 Jun 14; 5(6):e11115en_US
dc.identifier.issn1932-6203en_US
dc.identifier.pmid20559439en_US
dc.identifier.doi10.1371/journal.pone.0011115en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/587-
dc.description.abstractBackground: Neurexin is a synaptic cell adhesion protein critical for synapse formation and function. Mutations in neurexin and neurexin-interacting proteins have been implicated in several neurological diseases. Previous studies have described Drosophila neurexin mutant phenotypes in third instar larvae and adults. However, the expression and function of Drosophila neurexin early in synapse development, when neurexin function is thought to be most important, has not been described.en_US
dc.description.abstractMethodology/Principal Findings: We use a variety of techniques, including immunohistochemistry, electron microscopy, in situ hybridization, and electrophysiology, to characterize neurexin expression and phenotypes in embryonic Drosophila neuromuscular junctions (NMJs). Our results surprisingly suggest that neurexin in embryos is present both pre and postsynaptically. Presynaptic neurexin promotes presynaptic active zone formation and neurotransmitter release, but along with postsynaptic neurexin, also suppresses formation of ectopic glutamate receptor clusters. Interestingly, we find that loss of neurexin only affects receptors containing the subunit GluRIIA.en_US
dc.description.abstractConclusions/Significance: Our study extends previous results and provides important detail regarding the role of neurexin in Drosophila glutamate receptor abundance. The possibility that neurexin is present postsynaptically raises new hypotheses regarding neurexin function in synapses, and our results provide new insights into the role of neurexin in synapse development.en_US
dc.rightsChen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.subjectResearch Articleen_US
dc.subjectNeuroscience/Neurodevelopmenten_US
dc.subjectNeuroscience/Neuronal and Glial Cell Biologyen_US
dc.subjectNeuroscience/Neuronal Signaling Mechanismsen_US
dc.titleNeurexin in Embryonic Drosophila Neuromuscular Junctionsen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC2885429en_US
dc.contributor.corporatenameDepartment of Neurology-
dc.contributor.corporatenameCollege of Graduate Studies-

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