beta-Catenin Regulates Intercellular Signalling Networks and Cell-Type Specific Transcription in the Developing Mouse Midbrain-Rhombomere 1 Region

Hdl Handle:
http://hdl.handle.net/10675.2/586
Title:
beta-Catenin Regulates Intercellular Signalling Networks and Cell-Type Specific Transcription in the Developing Mouse Midbrain-Rhombomere 1 Region
Authors:
Chilov, Dmitri ( 0000-0002-2279-0854 ) ; Sinjushina, Natalia; Saarimaki-Vire, Jonna; Taketo, Makoto M.; Partanen, Juha
Abstract:
b-catenin is a multifunctional protein involved in both signalling by secreted factors of Wnt family and regulation of the cellular architecture. We show that b-catenin stabilization in mouse midbrain-rhombomere1 region leads to robust upregulation of several Wnt signalling target genes, including Fgf8. Suggestive of direct transcriptional regulation of the Fgf8 gene, b-catenin stabilization resulted in Fgf8 up-regulation also in other tissues, specifically in the ventral limb ectoderm. Interestingly, stabilization of b-catenin rapidly caused down-regulation of the expression of Wnt1 itself, suggesting a negative feedback loop. The changes in signal molecule expression were concomitant with deregulation of anteriorposterior and dorso-ventral patterning. The transcriptional regulatory functions of b-catenin were confirmed by b-catenin loss-of-function experiments. Temporally controlled inactivation of b-catenin revealed a cell-autonomous role for b-catenin in the maintenance of cell-type specific gene expression in the progenitors of midbrain dopaminergic neurons. These results highlight the role of b-catenin in establishment of neuroectodermal signalling centers, promoting region-specific gene expression and regulation of cell fate determination.
Editors:
Mei, Lin
Citation:
PLoS One. 2010 Jun 3; 5(6):e10881
Issue Date:
3-Jun-2010
URI:
http://hdl.handle.net/10675.2/586
DOI:
10.1371/journal.pone.0010881
PubMed ID:
20532162
PubMed Central ID:
PMC2880587
Type:
Article
ISSN:
1932-6203
Appears in Collections:
Department of Neurology: Faculty Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorChilov, Dmitrien_US
dc.contributor.authorSinjushina, Nataliaen_US
dc.contributor.authorSaarimaki-Vire, Jonnaen_US
dc.contributor.authorTaketo, Makoto M.en_US
dc.contributor.authorPartanen, Juhaen_US
dc.contributor.editorMei, Lin-
dc.date.accessioned2012-10-26T16:26:48Z-
dc.date.available2012-10-26T16:26:48Z-
dc.date.issued2010-06-3en_US
dc.identifier.citationPLoS One. 2010 Jun 3; 5(6):e10881en_US
dc.identifier.issn1932-6203en_US
dc.identifier.pmid20532162en_US
dc.identifier.doi10.1371/journal.pone.0010881en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/586-
dc.description.abstractb-catenin is a multifunctional protein involved in both signalling by secreted factors of Wnt family and regulation of the cellular architecture. We show that b-catenin stabilization in mouse midbrain-rhombomere1 region leads to robust upregulation of several Wnt signalling target genes, including Fgf8. Suggestive of direct transcriptional regulation of the Fgf8 gene, b-catenin stabilization resulted in Fgf8 up-regulation also in other tissues, specifically in the ventral limb ectoderm. Interestingly, stabilization of b-catenin rapidly caused down-regulation of the expression of Wnt1 itself, suggesting a negative feedback loop. The changes in signal molecule expression were concomitant with deregulation of anteriorposterior and dorso-ventral patterning. The transcriptional regulatory functions of b-catenin were confirmed by b-catenin loss-of-function experiments. Temporally controlled inactivation of b-catenin revealed a cell-autonomous role for b-catenin in the maintenance of cell-type specific gene expression in the progenitors of midbrain dopaminergic neurons. These results highlight the role of b-catenin in establishment of neuroectodermal signalling centers, promoting region-specific gene expression and regulation of cell fate determination.en_US
dc.rightsChilov et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.subjectResearch Articleen_US
dc.subjectDevelopmental Biology/Developmental Molecular Mechanismsen_US
dc.subjectDevelopmental Biology/Molecular Developmenten_US
dc.subjectDevelopmental Biology/Neurodevelopmenten_US
dc.subjectDevelopmental Biology/Pattern Formationen_US
dc.titlebeta-Catenin Regulates Intercellular Signalling Networks and Cell-Type Specific Transcription in the Developing Mouse Midbrain-Rhombomere 1 Regionen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC2880587en_US
dc.contributor.corporatenameDepartment of Neurology-

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