Regulation of Adipose Tissue Stromal Cells Behaviors by Endogenic Oct4 Expression Control

Hdl Handle:
http://hdl.handle.net/10675.2/573
Title:
Regulation of Adipose Tissue Stromal Cells Behaviors by Endogenic Oct4 Expression Control
Authors:
Kim, Jung Hwan; Jee, Min Ki; Lee, So Young; Han, Tae Hee ( 0000-0002-9063-4052 ) ; Kim, Bong Sun; Kang, Kyung Sun; Kang, Soo Kyung
Abstract:
Background: To clarify the role of the POU domain transcription factor Oct4 in Adipose Tissue Stromal Cells (ATSCs), we investigated the regulation of Oct4 expression and other embryonic genes in fully differentiated cells, in addition to identifying expression at the gene and protein levels. The ATSCs and several immature cells were routinely expressing Oct4 protein before and after differentiating into specific lineages.; Methodology/Principal Findings and Conclusions: Here, we demonstrated the role of Oct4 in ATSCs on cell proliferation and differentiation. Exogenous Oct4 improves adult ATSCs cell proliferation and differentiation potencies through epigenetic reprogramming of stemness genes such as Oct4, Nanog, Sox2, and Rex1. Oct4 directly or indirectly induces ATSCs reprogramming along with the activation of JAK/STAT3 and ERK1/2. Exogenic Oct4 introduced a transdifferentiation priority into the neural lineage than mesodermal lineage. Global gene expression analysis results showed that Oct4 regulated target genes which could be characterized as differentially regulated genes such as pluripotency markers NANOG, SOX2, and KLF4 and markers of undifferentiated stem cells FOXD1, CDC2, and EPHB1. The negatively regulated genes included FAS, TNFR, COL6A1, JAM2, FOXQ1, FOXO1, NESTIN, SMAD3, SLIT3, DKK1, WNT5A, BMP1, and GLIS3 which are implicated in differentiation processes as well as a number of novel genes. Finally we have demonstrated the therapeutic utility of Oct4/ATSCs were introduced into the mouse traumatic brain, engrafted cells was more effectively induces regeneration activity with high therapeutic modality than that of control ATSCs. Engrafted Oct4/ATSCs efficiently migrated and transdifferentiated into action potential carrying, functionally neurons in the hippocampus and promoting the amelioration of lesion cavities.
Editors:
Mei, Lin
Citation:
PLoS One. 2009 Sep 24; 4(9):e7166
Issue Date:
24-Sep-2009
URI:
http://hdl.handle.net/10675.2/573
DOI:
10.1371/journal.pone.0007166
PubMed ID:
19777066
PubMed Central ID:
PMC2747014
Type:
Article
ISSN:
1932-6203
Appears in Collections:
Department of Neurology: Faculty Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorKim, Jung Hwanen_US
dc.contributor.authorJee, Min Kien_US
dc.contributor.authorLee, So Youngen_US
dc.contributor.authorHan, Tae Heeen_US
dc.contributor.authorKim, Bong Sunen_US
dc.contributor.authorKang, Kyung Sunen_US
dc.contributor.authorKang, Soo Kyungen_US
dc.contributor.editorMei, Lin-
dc.date.accessioned2012-10-26T16:26:45Z-
dc.date.available2012-10-26T16:26:45Z-
dc.date.issued2009-09-24en_US
dc.identifier.citationPLoS One. 2009 Sep 24; 4(9):e7166en_US
dc.identifier.issn1932-6203en_US
dc.identifier.pmid19777066en_US
dc.identifier.doi10.1371/journal.pone.0007166en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/573-
dc.description.abstractBackground: To clarify the role of the POU domain transcription factor Oct4 in Adipose Tissue Stromal Cells (ATSCs), we investigated the regulation of Oct4 expression and other embryonic genes in fully differentiated cells, in addition to identifying expression at the gene and protein levels. The ATSCs and several immature cells were routinely expressing Oct4 protein before and after differentiating into specific lineages.en_US
dc.description.abstractMethodology/Principal Findings and Conclusions: Here, we demonstrated the role of Oct4 in ATSCs on cell proliferation and differentiation. Exogenous Oct4 improves adult ATSCs cell proliferation and differentiation potencies through epigenetic reprogramming of stemness genes such as Oct4, Nanog, Sox2, and Rex1. Oct4 directly or indirectly induces ATSCs reprogramming along with the activation of JAK/STAT3 and ERK1/2. Exogenic Oct4 introduced a transdifferentiation priority into the neural lineage than mesodermal lineage. Global gene expression analysis results showed that Oct4 regulated target genes which could be characterized as differentially regulated genes such as pluripotency markers NANOG, SOX2, and KLF4 and markers of undifferentiated stem cells FOXD1, CDC2, and EPHB1. The negatively regulated genes included FAS, TNFR, COL6A1, JAM2, FOXQ1, FOXO1, NESTIN, SMAD3, SLIT3, DKK1, WNT5A, BMP1, and GLIS3 which are implicated in differentiation processes as well as a number of novel genes. Finally we have demonstrated the therapeutic utility of Oct4/ATSCs were introduced into the mouse traumatic brain, engrafted cells was more effectively induces regeneration activity with high therapeutic modality than that of control ATSCs. Engrafted Oct4/ATSCs efficiently migrated and transdifferentiated into action potential carrying, functionally neurons in the hippocampus and promoting the amelioration of lesion cavities.en_US
dc.rightsKim et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.subjectResearch Articleen_US
dc.subjectCell Biology/Cell Growth and Divisionen_US
dc.subjectCell Biology/Gene Expressionen_US
dc.subjectDevelopmental Biology/Cell Differentiationen_US
dc.subjectDevelopmental Biology/Stem Cellsen_US
dc.titleRegulation of Adipose Tissue Stromal Cells Behaviors by Endogenic Oct4 Expression Controlen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC2747014en_US
dc.contributor.corporatenameDepartment of Neurology-
dc.contributor.corporatenameCollege of Graduate Studies-

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