A Novel Function of RING Finger Protein 10 in Transcriptional Regulation of the Myelin-Associated Glycoprotein Gene and Myelin Formation in Schwann Cells

Hdl Handle:
http://hdl.handle.net/10675.2/567
Title:
A Novel Function of RING Finger Protein 10 in Transcriptional Regulation of the Myelin-Associated Glycoprotein Gene and Myelin Formation in Schwann Cells
Authors:
Hoshikawa, Shinya; Ogata, Toru; Fujiwara, Sayaka; Nakamura, Kozo; Tanaka, Sakae
Abstract:
Myelin-associated glycoprotein (MAG) has been detected in Schwann cells prior to the onset of myelination, suggesting its functions in the initiation of myelination. However, transcriptional regulatory mechanisms of MAG remain to be elucidated. Here, we analyzed the promoter of the MAG gene by using luciferase reporter systems in the primary rat Schwann cells. We identified a novel cis-acting element located 160 bp upstream from the MAG transcription initiation site. Using the identified cis-element as a bait, we performed yeast one-hybrid screening and isolated a cDNA encoding a RNF10 as a putative trans-acting protein. When overexpressed in Schwann cells, RNF10 enhanced the activity of the MAG promoter. When RNF10 expression in Schwann cells was knocked down by siRNA, endogenous MAG mRNA and protein expression decreased. Furthermore, we evaluated myelin synthesis using Schwann cell-DRG neuron cocultures. When Schwann cells were infected with retrovirus expressing RNF10 siRNA, myelin formation was inhibited. These data suggest that RNF10 regulates MAG expression and is required for myelin formation.
Editors:
Mei, Lin
Citation:
PLoS ONE. 2008 Oct 21; 3(10):e3464
Issue Date:
21-Oct-2008
URI:
http://hdl.handle.net/10675.2/567
DOI:
10.1371/journal.pone.0003464
PubMed ID:
18941509
PubMed Central ID:
PMC2565066
Type:
Article
ISSN:
1932-6203
Appears in Collections:
Department of Neurology: Faculty Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorHoshikawa, Shinyaen_US
dc.contributor.authorOgata, Toruen_US
dc.contributor.authorFujiwara, Sayakaen_US
dc.contributor.authorNakamura, Kozoen_US
dc.contributor.authorTanaka, Sakaeen_US
dc.contributor.editorMei, Lin-
dc.date.accessioned2012-10-26T16:26:42Z-
dc.date.available2012-10-26T16:26:42Z-
dc.date.issued2008-10-21en_US
dc.identifier.citationPLoS ONE. 2008 Oct 21; 3(10):e3464en_US
dc.identifier.issn1932-6203en_US
dc.identifier.pmid18941509en_US
dc.identifier.doi10.1371/journal.pone.0003464en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/567-
dc.description.abstractMyelin-associated glycoprotein (MAG) has been detected in Schwann cells prior to the onset of myelination, suggesting its functions in the initiation of myelination. However, transcriptional regulatory mechanisms of MAG remain to be elucidated. Here, we analyzed the promoter of the MAG gene by using luciferase reporter systems in the primary rat Schwann cells. We identified a novel cis-acting element located 160 bp upstream from the MAG transcription initiation site. Using the identified cis-element as a bait, we performed yeast one-hybrid screening and isolated a cDNA encoding a RNF10 as a putative trans-acting protein. When overexpressed in Schwann cells, RNF10 enhanced the activity of the MAG promoter. When RNF10 expression in Schwann cells was knocked down by siRNA, endogenous MAG mRNA and protein expression decreased. Furthermore, we evaluated myelin synthesis using Schwann cell-DRG neuron cocultures. When Schwann cells were infected with retrovirus expressing RNF10 siRNA, myelin formation was inhibited. These data suggest that RNF10 regulates MAG expression and is required for myelin formation.en_US
dc.rightsHoshikawa et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.subjectResearch Articleen_US
dc.subjectCell Biology/Gene Expressionen_US
dc.subjectCell Biology/Neuronal and Glial Cell Biologyen_US
dc.subjectNeuroscience/Neurodevelopmenten_US
dc.subjectNeuroscience/Neuronal and Glial Cell Biologyen_US
dc.titleA Novel Function of RING Finger Protein 10 in Transcriptional Regulation of the Myelin-Associated Glycoprotein Gene and Myelin Formation in Schwann Cellsen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC2565066en_US
dc.contributor.corporatenameDepartment of Neurology-
dc.contributor.corporatenameCollege of Graduate Studies-

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