Hdl Handle:
http://hdl.handle.net/10675.2/555
Title:
Critical Roles of Pten in B Cell Homeostasis and Immunoglobulin Class Switch Recombination
Authors:
Suzuki, Akira; Kaisho, Tsuneyasu; Ohishi, Minako; Tsukio-Yamaguchi, Manae; Tsubata, Takeshi; Koni, Pandelakis A.; Sasaki, Takehiko; Mak, Tak Wah; Nakano, Toru
Abstract:
Pten is a tumor suppressor gene mutated in human cancers. We used the Cre-loxP system to generate a B cellâ specific mutation of Pten in mice (bPtenflox/floxmice). bPtenflox/flox mice showed elevated numbers of B1a cells and increased serum autoantibodies. Among B2 cells in bPtenflox/flox spleens, numbers of marginal zone B (MZB) cells were significantly increased while those of follicular B (FOB) cells were correspondingly decreased. Pten-deficient B cells hyperproliferated, were resistant to apoptotic stimuli, and showed enhanced migration. The survival kinase PKB/Akt was highly activated in Pten-deficient splenic B cells. In addition, immunoglobulin class switch recombination was defective and induction of activation-induced cytidine deaminase (AID) was impaired. Thus, Pten plays a role in developmental fate determination of B cells and is an indispensable regulator of B cell homeostasis.
Citation:
J Exp Med. 2003 Mar 3; 197(5):657-667
Issue Date:
3-Mar-2003
URI:
http://hdl.handle.net/10675.2/555
DOI:
10.1084/jem.20021101
PubMed ID:
12615906
PubMed Central ID:
PMC2193827
Type:
Article
ISSN:
1540-9538
Appears in Collections:
Institute of Molecular Medicine and Genetics: Faculty Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorSuzuki, Akiraen_US
dc.contributor.authorKaisho, Tsuneyasuen_US
dc.contributor.authorOhishi, Minakoen_US
dc.contributor.authorTsukio-Yamaguchi, Manaeen_US
dc.contributor.authorTsubata, Takeshien_US
dc.contributor.authorKoni, Pandelakis A.en_US
dc.contributor.authorSasaki, Takehikoen_US
dc.contributor.authorMak, Tak Wahen_US
dc.contributor.authorNakano, Toruen_US
dc.date.accessioned2012-10-26T16:26:39Z-
dc.date.available2012-10-26T16:26:39Z-
dc.date.issued2003-03-3en_US
dc.identifier.citationJ Exp Med. 2003 Mar 3; 197(5):657-667en_US
dc.identifier.issn1540-9538en_US
dc.identifier.pmid12615906en_US
dc.identifier.doi10.1084/jem.20021101en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/555-
dc.description.abstractPten is a tumor suppressor gene mutated in human cancers. We used the Cre-loxP system to generate a B cellâ specific mutation of Pten in mice (bPtenflox/floxmice). bPtenflox/flox mice showed elevated numbers of B1a cells and increased serum autoantibodies. Among B2 cells in bPtenflox/flox spleens, numbers of marginal zone B (MZB) cells were significantly increased while those of follicular B (FOB) cells were correspondingly decreased. Pten-deficient B cells hyperproliferated, were resistant to apoptotic stimuli, and showed enhanced migration. The survival kinase PKB/Akt was highly activated in Pten-deficient splenic B cells. In addition, immunoglobulin class switch recombination was defective and induction of activation-induced cytidine deaminase (AID) was impaired. Thus, Pten plays a role in developmental fate determination of B cells and is an indispensable regulator of B cell homeostasis.en_US
dc.rightsCopyright © 2003, The Rockefeller University Pressen_US
dc.titleCritical Roles of Pten in B Cell Homeostasis and Immunoglobulin Class Switch Recombinationen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC2193827en_US
dc.contributor.corporatenameInstitute of Molecular Medicine and Genetics-

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