Hdl Handle:
http://hdl.handle.net/10675.2/553
Title:
Conditional Vascular Cell Adhesion Molecule 1 Deletion in Mice
Authors:
Koni, Pandelakis A.; Joshi, Sunil K.; Temann, Ulla-Angela; Olson, Dian; Burkly, Linda; Flavell, Richard A. ( 0000-0003-4461-0778 )
Abstract:
We generated vascular cell adhesion molecule (VCAM)-1 “knock-in” mice and Cre recombinase transgenic mice to delete the VCAM-1 gene ( vcam-1 ) in whole mice, thereby overcoming the embryonic lethality seen with conventional vcam-1 –deficient mice. vcam-1 knock-in mice expressed normal levels of VCAM-1 but showed loss of VCAM-1 on endothelial and hematopoietic cells when interbred with a “TIE2Cre” transgene. Analysis of peripheral blood from conditional vcam-1– deficient mice revealed mild leukocytosis, including elevated immature B cell numbers. Conversely, the bone marrow (BM) had reduced immature B cell numbers, but normal numbers of pro-B cells. vcam-1 –deficient mice also had reduced mature IgD 1 B and T cells in BM and a greatly reduced capacity to support short-term migration of transferred B cells, CD4 1 T cells, CD8 1 T cells, and preactivated CD4 1 T cells to the BM. Thus, we report an until now unappreciated dominant role for VCAM-1 in lymphocyte homing to BM.
Citation:
J Exp Med. 2001 Mar 19; 193(6):741-754
Issue Date:
19-Mar-2001
URI:
http://hdl.handle.net/10675.2/553
PubMed ID:
11257140
PubMed Central ID:
PMC2193418
Type:
Article
ISSN:
1540-9538
Appears in Collections:
Institute of Molecular Medicine and Genetics: Faculty Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorKoni, Pandelakis A.en_US
dc.contributor.authorJoshi, Sunil K.en_US
dc.contributor.authorTemann, Ulla-Angelaen_US
dc.contributor.authorOlson, Dianen_US
dc.contributor.authorBurkly, Lindaen_US
dc.contributor.authorFlavell, Richard A.en_US
dc.date.accessioned2012-10-26T16:26:38Z-
dc.date.available2012-10-26T16:26:38Z-
dc.date.issued2001-03-19en_US
dc.identifier.citationJ Exp Med. 2001 Mar 19; 193(6):741-754en_US
dc.identifier.issn1540-9538en_US
dc.identifier.pmid11257140en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/553-
dc.description.abstractWe generated vascular cell adhesion molecule (VCAM)-1 “knock-in” mice and Cre recombinase transgenic mice to delete the VCAM-1 gene ( vcam-1 ) in whole mice, thereby overcoming the embryonic lethality seen with conventional vcam-1 –deficient mice. vcam-1 knock-in mice expressed normal levels of VCAM-1 but showed loss of VCAM-1 on endothelial and hematopoietic cells when interbred with a “TIE2Cre” transgene. Analysis of peripheral blood from conditional vcam-1– deficient mice revealed mild leukocytosis, including elevated immature B cell numbers. Conversely, the bone marrow (BM) had reduced immature B cell numbers, but normal numbers of pro-B cells. vcam-1 –deficient mice also had reduced mature IgD 1 B and T cells in BM and a greatly reduced capacity to support short-term migration of transferred B cells, CD4 1 T cells, CD8 1 T cells, and preactivated CD4 1 T cells to the BM. Thus, we report an until now unappreciated dominant role for VCAM-1 in lymphocyte homing to BM.en_US
dc.rights© 2001 The Rockefeller University Pressen_US
dc.subjectOriginal Articleen_US
dc.titleConditional Vascular Cell Adhesion Molecule 1 Deletion in Miceen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC2193418en_US
dc.contributor.corporatenameInstitute of Molecular Medicine and Genetics-
dc.contributor.corporatenameDepartment of Medicine-
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