Hdl Handle:
http://hdl.handle.net/10675.2/549
Title:
BDNF-induced recruitment of TrkB receptor into neuronal lipid rafts
Authors:
Suzuki, Shingo; Numakawa, Tadahiro; Shimazu, Kazuhiro; Koshimizu, Hisatsugu; Hara, Tomoko; Hatanaka, Hiroshi; Mei, Lin; Lu, Bai; Kojima, Masami
Abstract:
Brain-derived neurotrophic factor (BDNF) plays an important role in synaptic plasticity but the underlying signaling mechanisms remain unknown. Here, we show that BDNF rapidly recruits full-length TrkB (TrkB-FL) receptor into cholesterol-rich lipid rafts from nonraft regions of neuronal plasma membranes. Translocation of TrkB-FL was blocked by Trk inhibitors, suggesting a role of TrkB tyrosine kinase in the translocation. Disruption of lipid rafts by depleting cholesterol from cell surface blocked the ligand-induced translocation. Moreover, disruption of lipid rafts prevented potentiating effects of BDNF on transmitter release in cultured neurons and synaptic response to tetanus in hippocampal slices. In contrast, lipid rafts are not required for BDNF regulation of neuronal survival. Thus, ligand-induced TrkB translocation into lipid rafts may represent a signaling mechanism selective for synaptic modulation by BDNF in the central nervous system.
Citation:
J Cell Biol. 2004 Dec 20; 167(6):1205-1215
Issue Date:
20-Dec-2004
URI:
http://hdl.handle.net/10675.2/549
DOI:
10.1083/jcb.200404106
PubMed ID:
15596541
PubMed Central ID:
PMC2172613
Type:
Article
ISSN:
1540-8140
Appears in Collections:
Institute of Molecular Medicine and Genetics: Faculty Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorSuzuki, Shingoen_US
dc.contributor.authorNumakawa, Tadahiroen_US
dc.contributor.authorShimazu, Kazuhiroen_US
dc.contributor.authorKoshimizu, Hisatsuguen_US
dc.contributor.authorHara, Tomokoen_US
dc.contributor.authorHatanaka, Hiroshien_US
dc.contributor.authorMei, Linen_US
dc.contributor.authorLu, Baien_US
dc.contributor.authorKojima, Masamien_US
dc.date.accessioned2012-10-26T16:26:37Z-
dc.date.available2012-10-26T16:26:37Z-
dc.date.issued2004-12-20en_US
dc.identifier.citationJ Cell Biol. 2004 Dec 20; 167(6):1205-1215en_US
dc.identifier.issn1540-8140en_US
dc.identifier.pmid15596541en_US
dc.identifier.doi10.1083/jcb.200404106en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/549-
dc.description.abstractBrain-derived neurotrophic factor (BDNF) plays an important role in synaptic plasticity but the underlying signaling mechanisms remain unknown. Here, we show that BDNF rapidly recruits full-length TrkB (TrkB-FL) receptor into cholesterol-rich lipid rafts from nonraft regions of neuronal plasma membranes. Translocation of TrkB-FL was blocked by Trk inhibitors, suggesting a role of TrkB tyrosine kinase in the translocation. Disruption of lipid rafts by depleting cholesterol from cell surface blocked the ligand-induced translocation. Moreover, disruption of lipid rafts prevented potentiating effects of BDNF on transmitter release in cultured neurons and synaptic response to tetanus in hippocampal slices. In contrast, lipid rafts are not required for BDNF regulation of neuronal survival. Thus, ligand-induced TrkB translocation into lipid rafts may represent a signaling mechanism selective for synaptic modulation by BDNF in the central nervous system.en_US
dc.rightsCopyright © 2004, Governmenten_US
dc.titleBDNF-induced recruitment of TrkB receptor into neuronal lipid raftsen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC2172613en_US
dc.contributor.corporatenameInstitute of Molecular Medicine and Genetics-
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