Hdl Handle:
http://hdl.handle.net/10675.2/541
Title:
PROTEIN SYNTHESIS IN PANCREAS OF FASTED PIGEONS
Authors:
Black, Owen; Webster, Paul D.
Abstract:
The regulation of protein synthesis in the pigeon has been studied by comparing the capability of cell-free amino acid incorporating systems of membrane-bound and membrane-free polysomes prepared from fasted and fed birds. New methods were developed for isolating polysomes since techniques used for other tissues did not provide quantitative recovery of polysomal RNA. The sucrose gradient profile of polysomes from pigeon pancreas showed a predominance of trisome species. Although initiation factors are present on polysomes, it was found that polysomes in cell-free systems would not initiate protein synthesis without exogenous initiation factors. This suggested the presence of an inhibitor or regulator of protein synthesis. These studies show that fasting resulted in: (a) decreased amounts of polysomes; (b) disaggregation of polysomes to monosomes; (c) decreased capability of polysomes to synthesize nascent peptides and to initiate additional synthesis, apparently not related to concentration of initiation factors.
Citation:
J Cell Biol. 1973 Apr 1; 57(1):1-8
Issue Date:
1-Apr-1973
URI:
http://hdl.handle.net/10675.2/541
PubMed ID:
4691394
PubMed Central ID:
PMC2108954
Type:
Article
ISSN:
1540-8140
Appears in Collections:
Department of Medicine Faculty: Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorBlack, Owenen_US
dc.contributor.authorWebster, Paul D.en_US
dc.date.accessioned2012-10-26T16:26:35Z-
dc.date.available2012-10-26T16:26:35Z-
dc.date.issued1973-04-1en_US
dc.identifier.citationJ Cell Biol. 1973 Apr 1; 57(1):1-8en_US
dc.identifier.issn1540-8140en_US
dc.identifier.pmid4691394en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/541-
dc.description.abstractThe regulation of protein synthesis in the pigeon has been studied by comparing the capability of cell-free amino acid incorporating systems of membrane-bound and membrane-free polysomes prepared from fasted and fed birds. New methods were developed for isolating polysomes since techniques used for other tissues did not provide quantitative recovery of polysomal RNA. The sucrose gradient profile of polysomes from pigeon pancreas showed a predominance of trisome species. Although initiation factors are present on polysomes, it was found that polysomes in cell-free systems would not initiate protein synthesis without exogenous initiation factors. This suggested the presence of an inhibitor or regulator of protein synthesis. These studies show that fasting resulted in: (a) decreased amounts of polysomes; (b) disaggregation of polysomes to monosomes; (c) decreased capability of polysomes to synthesize nascent peptides and to initiate additional synthesis, apparently not related to concentration of initiation factors.en_US
dc.rightsCopyright © 1973 by The Rockefeller University Pressen_US
dc.titlePROTEIN SYNTHESIS IN PANCREAS OF FASTED PIGEONSen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC2108954en_US
dc.contributor.corporatenameDepartment of Medicine-
dc.contributor.corporatenameDepartment of Cellular Biology and Anatomy-
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