Properties of the Force Exerted by Filopodia and Lamellipodia and the Involvement of Cytoskeletal Components

Hdl Handle:
http://hdl.handle.net/10675.2/538
Title:
Properties of the Force Exerted by Filopodia and Lamellipodia and the Involvement of Cytoskeletal Components
Authors:
Cojoc, Dan; Difato, Francesco ( 0000-0001-6404-588X ) ; Ferrari, Enrico ( 0000-0002-9102-7422 ) ; Shahapure, Rajesh B.; Laishram, Jummi; Righi, Massimo; Di Fabrizio, Enzo M.; Torre, Vincent
Abstract:
During neuronal differentiation, lamellipodia and filopodia explore the environment in search for the correct path to the axon's final destination. Although the motion of lamellipodia and filopodia has been characterized to an extent, little is known about the force they exert. In this study, we used optical tweezers to measure the force exerted by filopodia and lamellipodia with a millisecond temporal resolution. We found that a single filopodium exerts a force not exceeding 3 pN, whereas lamellipodia can exert a force up to 20 pN. Using metabolic inhibitors, we showed that no force is produced in the absence of actin polymerization and that development of forces larger than 3 pN requires microtubule polymerization. These results show that actin polymerization is necessary for force production and demonstrate that not only do neurons process information, but they also act on their environment exerting forces varying from tenths pN to tens of pN.
Editors:
Mei, Lin
Citation:
PLoS ONE. 2007 Oct 24; 2(10):e1072
Issue Date:
24-Oct-2007
URI:
http://hdl.handle.net/10675.2/538
DOI:
10.1371/journal.pone.0001072
PubMed ID:
17957254
PubMed Central ID:
PMC2034605
Type:
Article
ISSN:
1932-6203
Appears in Collections:
Department of Neurology: Faculty Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorCojoc, Danen_US
dc.contributor.authorDifato, Francescoen_US
dc.contributor.authorFerrari, Enricoen_US
dc.contributor.authorShahapure, Rajesh B.en_US
dc.contributor.authorLaishram, Jummien_US
dc.contributor.authorRighi, Massimoen_US
dc.contributor.authorDi Fabrizio, Enzo M.en_US
dc.contributor.authorTorre, Vincenten_US
dc.contributor.editorMei, Lin-
dc.date.accessioned2012-10-26T16:26:34Z-
dc.date.available2012-10-26T16:26:34Z-
dc.date.issued2007-10-24en_US
dc.identifier.citationPLoS ONE. 2007 Oct 24; 2(10):e1072en_US
dc.identifier.issn1932-6203en_US
dc.identifier.pmid17957254en_US
dc.identifier.doi10.1371/journal.pone.0001072en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/538-
dc.description.abstractDuring neuronal differentiation, lamellipodia and filopodia explore the environment in search for the correct path to the axon's final destination. Although the motion of lamellipodia and filopodia has been characterized to an extent, little is known about the force they exert. In this study, we used optical tweezers to measure the force exerted by filopodia and lamellipodia with a millisecond temporal resolution. We found that a single filopodium exerts a force not exceeding 3 pN, whereas lamellipodia can exert a force up to 20 pN. Using metabolic inhibitors, we showed that no force is produced in the absence of actin polymerization and that development of forces larger than 3 pN requires microtubule polymerization. These results show that actin polymerization is necessary for force production and demonstrate that not only do neurons process information, but they also act on their environment exerting forces varying from tenths pN to tens of pN.en_US
dc.rightsCojoc et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.subjectResearch Articleen_US
dc.subjectBiophysicsen_US
dc.subjectNeuroscienceen_US
dc.subjectBiophysics/Experimental Biophysical Methodsen_US
dc.subjectCell Biology/Cytoskeletonen_US
dc.subjectDevelopmental Biology/Neurodevelopmenten_US
dc.subjectNeuroscience/Neurodevelopmenten_US
dc.titleProperties of the Force Exerted by Filopodia and Lamellipodia and the Involvement of Cytoskeletal Componentsen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC2034605en_US
dc.contributor.corporatenameDepartment of Neurology-
dc.contributor.corporatenameCollege of Graduate Studies-

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