Hdl Handle:
http://hdl.handle.net/10675.2/105
Title:
A facilitated tracking and transcription mechanism of long-range enhancer function.
Authors:
Zhu, Xingguo; Ling, Jianhua; Zhang, Ling; Pi, Wenhu; Wu, Min; Tuan, Dorothy
Abstract:
In the human epsilon-globin gene locus, the HS2 enhancer in the Locus Control Region regulates transcription of the embryonic epsilon-globin gene located over 10 kb away. The mechanism of long-range HS2 enhancer function was not fully established. Here we show that the HS2 enhancer complex containing the enhancer DNA together with RNA polymerase II (pol II) and TBP tracks along the intervening DNA, synthesizing short, polyadenylated, intergenic RNAs to ultimately loop with the epsilon-globin promoter. Guided by this facilitated tracking and transcription mechanism, the HS2 enhancer delivers pol II and TBP to the cis-linked globin promoter to activate mRNA synthesis from the target gene. An insulator inserted in the intervening DNA between the enhancer and the promoter traps the enhancer DNA and the associated pol II and TBP at the insulator site, blocking mid-stream the facilitated tracking and transcription mechanism of the enhancer complex, thereby blocking long-range enhancer function.
Citation:
Nucleic Acids Res. 2007 Aug 17; 35(16):5532-5544
Issue Date:
14-Sep-2007
URI:
http://hdl.handle.net/10675.2/105
DOI:
10.1093/nar/gkm595
PubMed ID:
17704132
PubMed Central ID:
PMC2018613
Type:
Journal Article; Research Support, N.I.H., Extramural
ISSN:
1362-4962
Appears in Collections:
Department of Biochemistry and Molecular Biology: Faculty Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorZhu, Xingguoen_US
dc.contributor.authorLing, Jianhuaen_US
dc.contributor.authorZhang, Lingen_US
dc.contributor.authorPi, Wenhuen_US
dc.contributor.authorWu, Minen_US
dc.contributor.authorTuan, Dorothyen_US
dc.date.accessioned2010-09-24T22:03:20Z-
dc.date.available2010-09-24T22:03:20Z-
dc.date.issued2007-09-14en_US
dc.identifier.citationNucleic Acids Res. 2007 Aug 17; 35(16):5532-5544en_US
dc.identifier.issn1362-4962en_US
dc.identifier.pmid17704132en_US
dc.identifier.doi10.1093/nar/gkm595en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/105-
dc.description.abstractIn the human epsilon-globin gene locus, the HS2 enhancer in the Locus Control Region regulates transcription of the embryonic epsilon-globin gene located over 10 kb away. The mechanism of long-range HS2 enhancer function was not fully established. Here we show that the HS2 enhancer complex containing the enhancer DNA together with RNA polymerase II (pol II) and TBP tracks along the intervening DNA, synthesizing short, polyadenylated, intergenic RNAs to ultimately loop with the epsilon-globin promoter. Guided by this facilitated tracking and transcription mechanism, the HS2 enhancer delivers pol II and TBP to the cis-linked globin promoter to activate mRNA synthesis from the target gene. An insulator inserted in the intervening DNA between the enhancer and the promoter traps the enhancer DNA and the associated pol II and TBP at the insulator site, blocking mid-stream the facilitated tracking and transcription mechanism of the enhancer complex, thereby blocking long-range enhancer function.en_US
dc.rightsThe PMC Open Access Subset is a relatively small part of the total collection of articles in PMC. Articles in the PMC Open Access Subset are still protected by copyright, but are made available under a Creative Commons or similar license that generally allows more liberal redistribution and reuse than a traditional copyrighted work. Please refer to the license statement in each article for specific terms of use. The license terms are not identical for all articles in this subset.en_US
dc.subject.meshAnimalsen_US
dc.subject.meshChickens / geneticsen_US
dc.subject.meshEnhancer Elements, Geneticen_US
dc.subject.meshGlobins / geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshInsulator Elementsen_US
dc.subject.meshK562 Cellsen_US
dc.subject.meshLocus Control Regionen_US
dc.subject.meshModels, Geneticen_US
dc.subject.meshNuclear Proteins / metabolismen_US
dc.subject.meshPolyadenylationen_US
dc.subject.meshPromoter Regions, Geneticen_US
dc.subject.meshRNA Polymerase II / metabolismen_US
dc.subject.meshTATA-Box Binding Protein / metabolismen_US
dc.subject.meshTranscription, Geneticen_US
dc.subject.meshZebrafish / geneticsen_US
dc.titleA facilitated tracking and transcription mechanism of long-range enhancer function.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, N.I.H., Extramuralen_US
dc.identifier.pmcidPMC2018613en_US
dc.contributor.corporatenameDepartment of Biochemistry and Molecular Biologyen_US
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